Enzymatic Detection of Urinary Steroids on Thin-Layer Chromatograms

Abstract

Enzymatic detection of urinary steroids on thin-layer chromatograms (TLC) is described. Steroids in urine such as neutral 3α-hydroxysteroids, 3β-hydroxysteroids, acidic 3α-hydroxysteroids and 17β-hydroxysteroids are detected on thin-layer plate using enzyme reactions.

Combination of chromatographic techniques and enzyme reactions give more specific analysis of the compounds in a body fluid. Some clinical application by the use of these enzymatic methods is also described and these enzymatic detection methods are shown to be useful for the diagnosis of some disease of abnormal steroid metabolism.