Carbohydrate Analysis by High Pressure Liquid Chromatography Using Water as the Eluent

Abstract

A μ-Bondapak C-18 column separates, by reverse phase process, mono, di and oligosaccharides within 40 min when water is used as eluent. The fractionation capabilities of this column are a function of experimental conditions (temperature and flow-rate). Chromatograms showing separations obtained for different carbohydrate mixtures are presented and discussed in terms of solubility parameters.