Separation and Identification of Labile Polyacetylenes by RP-HPLC

Abstract

Traditional methods of isolation and identification of labile polyacetylenes often lead to substantial loss of material. Reverse-phase HPLC coupled with a diode array detector offers a fast and convenient way to isolate and identify polyacetylenes from plant sources. Minimizing the number of manipulations reduces the potential for losses, and makes a more accurate quantitative analysis of the polyacetylenes possible.