Proposed Instrumental Spreading Shape Function (ISF) as Applied to Size Exclusion Chromatography (SEC)

Abstract

Herein is proposed a new statistical ISF. This function which is gaussian and symmetric in shape, is an infinite series. The series is described by two parameters, instrument peak dispersion coefficient, σ², and a new coefficient, a polyplatykurtic coefficient A_k. The two parameters combine to form a peak broadening parameter, X. This new shape function seems to fall within the expectation of several authors in the past (1–11). This series was obtained during studies involving aqueous SEC with series of dextran standards. This paper is a continuation of a series of papers on these investigations (12,13)