首页 | 本学科首页   官方微博 | 高级检索  
     


Analysis of Guanylate Cyclase Activity by High-Pressure Liquid Chromatography
Abstract:Abstract

A method for the assay of guanylate cyclase which permits the correction of concurrent phosphatase and phosphodiesterase reactions has been developed using HPLC. The method, based on the conversion of tritium labelled guanosine triphosphate to tritium labelled cyclic guanosine monophosphate, uses [14C]-cGMP as the internal standard to account for the degradative and procedural-losses. Radiolabelled reaction products are isolated by high pressure liquid chromatography on a Partisil SAX column with a single step isocratic elution using 12.5 mM potassium phosphate buffer (pH 3.25). Since column recovery of the nucleotides is virtually quantitative and complete purification is achieved, the method possesses a high degree of accuracy and precision.
Keywords:
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号