Determination of Methoprene in Water Samples by High Performance Liquid Chromatography

Abstract

A new high-performance liquid-chromatography method has been developed that can determine low concentrations of methoprene in water samples. The method allows for reliable detection of concentrations between 0.005 μg/mL and 0.5 μg/mL. The detection limit for 5-mL samples is 2.5 ng/mL at 255 nm. The standard curve is linear over the entire concentration range. The method was used to do a stability study on aqueous methoprene samples stored at room temperature and under refrigeration. The study shows that aqueous methoprene samples are stable when stored at 4°C for at least three weeks.