High Performance Liquid Chromatography of Low Molecular Weight Proteins on a Non-Ionic Macroreticular Polystyrene Resin

Abstract

A high performance liquid chromatography system is presented for analytical and preparative separation of proteins. The method utilizes a macroreticular polystyrene resin having no specific functional groups, and proteins are eluted by the use of linear gradient of acetonitrile (20% ?75%, v/v) in 0.1% (v/v) trifluoroacetic acid. In this standard elution system, twenty proteins having a molecular weight of 4,200–58,000 and an iso-electric point of 3.9–11.0 have been chromatographed successfully within 80 min. The method allows a rapid, sensitive, and high resolution separation of relatively low molecular weight proteins, where the isolated proteins can be used for subsequent biochemical determinations.