Determination of Thiamphenicol in Serum and Cerebrospinal Fluid with High-Pressure Liquid Chromatography

Abstract

A procedure for quantitation of thiamphenicol in serum and cerebrospinal fluid was developed using high-pressure liquid chromatography. The drug was extracted from biological samples with methanol and separated by reverse-phase high-pressure liquid chromatography. Detection and subsequent quantitation were performed at 254 nm by on-line ultraviolet spectrophotometry. After the intramuscular administration of a single dose of 1 g of thiamphenicol to a patient, a poor transmission of the drug across the hematoencephalic barrier was demonstrated by this assay.