Evidence for IFN-β heterogeneity in a substrain of namalwa cells |
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Authors: | H. Borg I. Nordensson T. Ny E. Lundgren |
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Affiliation: | 1. Unit for Applied Cell and Molecular Biology, University of Ume?, S-901 87, Ume?, Sweden
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Abstract: | A substrain of Namalwa cells, denoted substrain B, was grown in fermentors up to the 100-L scale, and was induced with Sendai virus to produce interferon (IFN). The titer of the crude IFN varied extensively between different batches; part of the variation was caused by a differential expression of IFN-alpha and IFN-beta. More than 80% of the IFN activity was IFN-beta by several criteria. A two-step purification procedure was developed and the resulting preparation had a specific activity of approximately 10(6) U/mg protein. The IFN-beta type was found to be heterogeneous, and could be separated into several components, which probably represented post-translational modifications of one molecule. |
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