Aspects of xylitol formation in sugarcane bagasse hydrolysate by Candida guillie rmondii in the presence of tetracycline |
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Authors: | Ernesto Acosta Martínez Silvio S Silva Maria G A Felipe |
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Institution: | (1) Department of Biotechnology, Faculty of Chemical Engineering of Lorena, Lorena, SP. Rod. Itajubá/Lorena Km 74,5, Caixa Postal 116, CEP 12600-000 Lorena, SP, Brasil;(2) Cuban Institute for Research on Sugarcane Derivatives, Havana City, Cuba |
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Abstract: | The biocon version of xylose intoxylitol using pH values of 4.0, 5.5 and 7.0 and tetracycline concentrations of 20 and 40
mg/L was carried out to verify the influence of these parameters on Candida guilliermondii metabolism for xylitol production. Experiments were performed with sugarcane bagasse hemicellulosi chydrolysate (48.5 g/L
of xylose) in 125-mL Erlenmeyer flasks, at 30°C, 200 rpm, during 88 h. The results demostrated that the bioconversion of xylose
into xylitol was significantly influenced by the pH. On the other hand, in media containing 20 or 40 mg/L of tetracycline,
this bioconversion was not significantly affected. The best results of xylitol production were obtained in hemicellulosic
hydrolysate without tetracycline, at pH 7.0 In these conditions, the maxim um specific growth rate was 0.014/h and the yield
factor of xylitol and volumetric productivity were 0.85g/g and 0.70g/L/h respectively. Xylitol and cell growth occureed simultaneously. |
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Keywords: | Hemicellulosic hydrolysate sugarcane bagasse xylose xylitol Candida guilliermondii Klebsiella pueumoniae |
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