Determination of morphine and 6-acetylmorphine in plasma by high-performance liquid chromatography with fluorescence detection |
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| Authors: | D A Barrett P N Shaw S S Davis |
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| Institution: | Department of Pharmaceutical Sciences, University Park, University of Nottingham, U.K. |
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| Abstract: | A method is described for the simultaneous determination of morphine and 6-acetylmorphine in small volumes of human plasma by normal-phase high-performance liquid chromatography using solid-phase extraction, dansyl derivatisation and fluorescence detection. The lower limits of quantitation in a 0.1-ml plasma sample are 10 ng/ml for morphine and 25 ng/ml for 6-acetylmorphine. The method has been applied to determine concentrations of morphine and 6-acetylmorphine in plasma samples from premature babies administered an intravenous infusion of diamorphine. |
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