Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging |
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| Authors: | Robinson Tom Valluri Prashant Manning Hugh B Owen Dylan M Munro Ian Talbot Clifford B Dunsby Christopher Eccleston John F Baldwin Geoff S Neil Mark A A de Mello Andrew J French Paul M W |
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| Institution: | Chemical Biology Centre, Department of Chemical Engineering, Imperial College London, London, UK. |
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| Abstract: | Fluorescence lifetime imaging (FLIM) is used to quantitatively map the concentration of a small molecule in three dimensions in a microfluidic mixing device. The resulting experimental data are compared with computational fluid-dynamics (CFD) simulations. A line-scanning semiconfocal FLIM microscope allows the full mixing profile to be imaged in a single scan with submicrometer resolution over an arbitrary channel length from the point of confluence. Following experimental and CFD optimization, mixing times down to 1.3+/-0.4 ms were achieved with the single-layer microfluidic device. |
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