Effects of Sphingomyelin-Containing Milk Phospholipids on Skin Hydration in UVB-Exposed Hairless Mice |
| |
Authors: | Yejin Ahn Min Guk Kim Kyungae Jo Ki-Bae Hong Hyung Joo Suh |
| |
Institution: | 1.Department of Integrated Biomedical and Life Science, Graduate School, Korea University, Seoul 02841, Korea; (Y.A.); (M.G.K.); (K.J.);2.Department of Food Science and Nutrition, Jeju National University, Jeju 63243, Korea;3.BK21FOUR R&E Center for Learning Health Systems, Korea University, Seoul 02841, Korea |
| |
Abstract: | Reactive oxygen species (ROS) generated by ultraviolet (UV) exposure cause skin barrier dysfunction, which leads to dry skin. In this study, the skin moisturizing effect of sphingomyelin-containing milk phospholipids in UV-induced hairless mice was evaluated. Hairless mice were irradiated with UVB for eight weeks, and milk phospholipids (50, 100, and 150 mg/kg) were administered daily. Milk phospholipids suppressed UV-induced increase in erythema and skin thickness, decreased transepidermal water loss, and increased skin moisture. Milk phospholipids increased the expression of filaggrin, involucrin, and aquaporin3 (AQP3), which are skin moisture-related factors. Additionally, hyaluronic acid (HA) content in the skin tissue was maintained by regulating the expression of HA synthesis- and degradation-related enzymes. Milk phospholipids alleviated UV-induced decrease in the expression of the antioxidant enzymes superoxidase dismutase1 and 2, catalase, and glutathione peroxidase1. Moreover, ROS levels were reduced by regulating heme oxygenase-1 (HO-1), an ROS regulator, through milk phospholipid-mediated activation of nuclear factor erythroid-2-related factor 2 (Nrf2). Collectively, sphingomyelin-containing milk phospholipids contributed to moisturizing the skin by maintaining HA content and reducing ROS levels in UVB-irradiated hairless mice, thereby, minimizing damage to the skin barrier caused by photoaging. |
| |
Keywords: | milk phospholipids sphingomyelin skin hydration Nrf2 hyaluronic acid |
|
|