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Application of enzyme-field effect transistor sensor arrays as detectors in a flow-injection analysis system for simultaneous monitoring of medium components. Part II. Monitoring of cultivation processes |
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| Authors: | T. Kullick, U. Bock, J. Schubert, T. Scheper,K. Schü gerl, |
| Affiliation: | a Institute for Technical Chemistry, University of Hannover, Callinstrasse 3, D-30167, Hannover, Germany b Institute of Biochemistry, Westfälische-Wilhems University Münster, Wilhelm-Klemm Strasse 2, D-48149, Münster, Germany |
| Abstract: | Glucose, maltose, sucrose, lactose, ethanol and urea concentrations were monitored simultaneously during the cultivation of Escherichia coli and Saccharomyces cerevisiae by means of enzyme field effect transistors (EnFETs) applying glucose dehydrogenase (GDH), maltase (MAL)/GDH, invertase (INV)/GDH, β-galactosidase (β-GAL)/galactosedehydrogenase (GALDH), alcoholdehydrogenase (ADH)/aldehydedehydrogenase (ALDH), and urease. These enzymes were (co)immobilized on the pH sensitive gates of an eight-FET array. The FET array was integrated in a commercial FIA system. |
| Keywords: | Flow injection Sensors Field effect transistor (FET) Glucose dehydrogenase Maltase Invertase β-Galactosidase Galactose dehydrogenase Alcohol dehydrogenase Aldehyde dehydrogenase Urease |
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