In vitro sonodynamic cytotoxicity in regulated cavitation conditions |
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Authors: | El Maalouf Jhony Béra Jean-Christophe Alberti Laurent Cathignol Dominique Mestas Jean-Louis |
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Institution: | a INSERM, U556, Lyon F-69003; Université de Lyon, Lyon F-69003, France; Université de lyon 1, Lyon F-69003, France b INSERM, U590, Centre Léon Bérard France, Université Lyon 1, F-69008, France |
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Abstract: | Sonodynamic toxicity has always been linked to the cavitation phenomenon. In this work, sonodynamic effect with Photofrin® was evaluated with a new ultrasound device: a regulated cavitation generator. In this way, acoustic intensity was substituted with cavitation level as ultrasound parameter. Photofrin® potentiated significantly the cavitation cytotoxicity even for low setpoints where no inertial cavitation appeared. Therefore sonodynamic mechanism was principally mechanical, facilitated by the Photofrin® insertion in cellular cytoplasmic membranes. This assertion was also supported by the fact that sonodynamic cytotoxicity was independent from the Photofrin® presence or absence in the extracellular medium. Reproducible sonodynamic efficiency was perfectly obtained with this new regulated cavitation generator. |
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Keywords: | Sonodynamic therapy Cavitation Hematoporphyrin Ultrasound Cavitation regulation Cytotoxicity |
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