Coupling Electrochemical Adsorption and Long‐range Electron Transfer: Label‐free DNA Mismatch Detection with Ultramicroelectrode (UME) |
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Authors: | Nivedita Basu Thu Huong Ho Franois‐Xavier Guillon Yuanyuan Zhang Pascal Bigey Bhat Navakanta Fethi Bedioui Mathieu Lazerges |
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Institution: | Nivedita Basu,Thu Huong Ho,François‐Xavier Guillon,Yuanyuan Zhang,Pascal Bigey,Bhat Navakanta,Fethi Bedioui,Mathieu Lazerges |
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Abstract: | A new electrochemical hybridization transduction pathway, obtained by coupling electrochemical adsorption and long‐range electron transfer through double‐stranded DNA, was investigated using ultramicroelectrode (UME). The results show that long‐range electron transfer does not occurs exclusively throws well‐packed and organized self‐assembled DNA monolayers. This approach is used to investigate long‐range electron transfer properties of both single‐ and double‐ stranded short synthetic DNA and DNA plasmids. Single mismatch electrochemical detection protocol of non‐labelled short synthetic DNA, without heating or probe labelling, in a 10 minutes protocol, was in fine performed. |
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Keywords: | DNA Electrochemical Adsorption Long-Range Electron Transfer Mismatch Ultramicroelectrode |
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