| Abstract: | An alternative strategy for the label‐free electrochemical detection of DNA hypermethylation using a microelectrode array as an oligodinucleotide (ODN) detector is presented. It relies on the oligonucleotide dependent electrostatic affinity interaction firstly with unmethylated ODN and then follow‐up with methylated DNA. The methylated cytosine status is quantified by monitoring the relative change in the exchange current at the ODN‐detector before and after the bisulfite treated DNA samples. This novel aproach displays unique advantages such as small working volumes of the analytes, low damage to DNA samples, easy integration of oligonucleides on the detector and signal evaluation. |
