Disposable Biosensor Based on Amidase/CeO2/GNR Modified Inkjet‐printed CNT Electrodes‐droplet Based Paracetamol Detection in Biological Fluids for “Point‐of‐care” Applications |
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Authors: | Dalibor M Stankovi Milo Ognjanovi Milica Jovi Valentina Cupli Andreas Lesch Hubert H Girault Marija GavroviJankulovi Bratislav Anti |
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Institution: | Dalibor M. Stankovi?,Milo? Ognjanovi?,Milica Jovi?,Valentina Cupli?,Andreas Lesch,Hubert H. Girault,Marija Gavrovi??Jankulovi?,Bratislav Anti? |
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Abstract: | A disposable acetaminophen biosensor based on inkjet‐printed CNT electrodes (IJPCNT) modified with amidase/cerium dioxide@graphene nanoribbons composite was developed (ACeO2@GNR/IJPCNT). The enzyme amidase A was used for the first time as a recognition element. Inkjet‐printed CNT electrodes served as a basis for the construction of a biosensor that enables droplet detection using 5 μL sample volume. The biosensor showed high selectivity, sensitivity, a low detection limit of 0.18 μM and a wide working linear range from 1 to 100 μM. The proposed approach allows fast and reliable detection of acetaminophen in biological fluids with negligible matrix effect and remarkable reproducibility. |
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Keywords: | acetaminophen amidase biosensor cerium dioxide/graphene nanoribbons composite inkjet-printed sensors |
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