Preparative isolation and purification of lutein from the microalga chlorella vulgaris by high-speed counter-current chromatography |
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| Authors: | Li H B Chen F Zhang T Y Yang F Q Xu G Q |
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| Affiliation: | Department of Botany, The University of Hong Kong, China. |
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| Abstract: | High-speed counter-current chromatography (HSCCC) was applied to the isolation and purification of lutein from microalgae. Analytical HSCCC was used for the preliminary selection of a suitable solvent system composed of n-hexane-ethanol-water (4:3:1, v/v). Using the above solvent system, preparative HSCCC was successfully performed yielding lutein at 98% purity from 200 mg of the crude extract in a one-step separation. |
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