Surface charge of PLA microparticles in regulation of antigen loading,macrophage phagocytosis and activation,and immune effects in vitro |
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| Authors: | Yuying Liu, Xiaoming Chen, Lianyan Wang, Tingyuan Yang, Qipeng Yuan, Guanghui Ma |
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| Affiliation: | [1]National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; [2]State Key Laboratory of Chemical Resource Engineering, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China; [3]University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | The use of microparticles(MPs) as adjuvants has attracted increasing interest in vaccine delivery systems.Many physiochemical characteristics of MPs including hydrodynamic size,surface properties,and morphology can regulate the immune response.Surface charge is an important characteristic of MPs,but how it affects their adjuvanticity remains unknown.In this study,we prepared uniform-sized polylactide MPs coated with various polymers of different positive charge,and investigated how the surface charge affected antigen loading and macrophage phagocytosis and activation in vitro.A higher surface charge greatly enhanced antigen loading and antigen internalization into macrophages,promoted the expression of MHC 11 and CD80,and increased the secretion level of TNF-a.Taken together,these results indicated that surface charge was an important parameter for improving the adjuvanticity of MPs. |
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| Keywords: | Microparticle（MP） Macrophage Surface charge Antigen loading Phagocytosis Activation |
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