天花粉蛋白一级结构的修正及不同产地天花粉蛋白的研究

胰蛋白酶酶解天花粉蛋白, 用高效液相色谱分离酶解肽段, 用顺序仪测定其有关肽段的顺序。用羧肽酶A, B, Y测定了天花粉蛋白C-端和天花粉蛋白溴化氰降解肽CB1的C-端顺序, 修正了我们1985年测定的天花粉蛋白一级结构, 证明天花粉蛋白由246(7)氨基酸残基所组成, 除C-端微观不均一外, 与Collins结果一致。同时比较了芜湖产天花粉蛋白一级结构与平湖产的天花粉蛋白一级结构, 没有发现两者的一级结构有差别。

Revision of the primary structure of trichosanthin and study on the trichosanthin from different places of origin

The primary structure of trichosanthin has been revised by sequencing its peptides isolated by reverse-phase HPLC from the tryptic digest of the intact trichosanthin as well as by kinetic studying on the carboxypeptidease (CpA, CpB and CpY) degradation of trichosanthin and of CB-1, the CNBr-degrdn. fragment of trichosanthin. The revised amino acid sequence consists of 246(7) residues, the same as those reported by Collins (1990) except there was microheterogeneity at the C-terminus. No difference has been shown from the studies of the amino acid sequence of trichosanthin from different places of origin, Wuhu in Anhui province and Pinghu in Zhejiang province.