Urinary testosterone measurement by gas chromatography after solid-phase extraction and high-performance liquid chromatography. |
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Authors: | E Venturelli A Manzari A Cavalleri M Benzo G Secreto E Marubini |
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Institution: | National Cancer Institute, Endocrine Unit, Milan, Italy. |
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Abstract: | A method was developed for the rapid determination of testosterone in urine. The procedure consists of solid-phase extraction (SPE) followed by high-performance liquid chromatographic (HPLC) clean-up before gas chromatographic determination. Recovery was evaluated by adding 3H]testosterone (10(4) cpm) to urine samples; the mean recovery of radioactivity after SPE and HPLC was 82%. Precision was estimated by repeated measurement of testosterone in four different urine samples; the coefficient of variation was 7.9% (95% confidence limits 6.1-11.4%). Accuracy was evaluated by standard addition and dilution assays; a linear relationship was found between the expected and observed values (r2 = 0.982). The method is rapid, effective and suitable for routine analysis. |
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