Simple and Rapid Determination of Denaturants in Alcohol Formulations by Hydrophilic Interaction Chromatography |
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Authors: | Ž Daunoravičius I Juknaitė E Naujalis A Padarauskas |
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Institution: | (1) Department of Analytical and Environmental Chemistry, Vilnius University, Naugarduko 24, 03225 Vilnius, Lithuania;(2) Department of Chemistry, Vilnius Pedagogical University, Studentu St. 39, 08106 Vilnius, Lithuania;(3) Laboratory for Metrology in Chemistry, Semiconductor Physics Institute, A. Gostauto 11, 01108 Vilnius, Lithuania |
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Abstract: | A hydrophilic interaction chromatography (HILIC) technique has been developed and validated for determination of common denaturants
(denatonium benzoate, crystal violet and methylene blue) in denaturated alcohol formulations. Among the three different polar
stationary phases (i.e., aminopropyl, cyanoethyl and silica) studied the cyanoethyl phase provided much stronger retention
for the organic cations. It was shown that high efficiencies were reached only with anionic ion-pairing reagent that reduces
the interactions with the silanol groups. The anion ion-pairing strength under HILIC conditions was: acetate < formate < trifluoroacetate
< perchlorate. This study also investigated the effect of various experimental factors on the retention of the cyanoethyl
stationary phase, such as acetonitrile content, pH and ion-pairing anion concentration in the mobile phase. The separation
of three denaturants was achieved in about 8 min with a mobile phase containing 60% (v/v) acetonitrile and 10 mmol L−1 HClO4. The proposed method was validated and applied to the determination of danaturating agents in various Lithuanian denaturated
alcohol formulations. |
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Keywords: | Hydrophilic interaction chromatography Denaturants Alcohol formulations |
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