|
|||||
|
|
Imaging of Enzyme Activity by Electron Paramagnetic Resonance: Concept and Experiment Using a Paramagnetic Substrate of Alkaline Phosphatase |
|
| Authors: | Urikhan Sanzhaeva Xuan Xu Priyaankadevi Guggilapu Prof. Dr. Mark Tseytlin Prof. Dr. Valery V. Khramtsov Prof. Dr. Benoit Driesschaert |
| Affiliation: | 1. In vivo Multifunctional Magnetic Resonance (IMMR) Center, Robert C. Byrd Health Sciences Center, West Virginia University, Morgantown, West, Virginia, USA;2. Department of Biochemistry, West Virginia University, School of Medicine, Morgantown, WV, USA;3. Lane Department of Computer Science and Electrical Engineering, West Virginia University, Morgantown, WV, USA;4. Current address: Department of Pharmaceutical Sciences, West Virginia University, School of Pharmacy, Morgantown, WV, USA |
| Abstract: | Enzyme activities are well established biomarkers of many pathologies. Imaging enzyme activity directly in vivo may help to gain insight into the pathogenesis of various diseases but remains extremely challenging. In this communication, we report the use of EPR imaging (EPRI) in combination with a specially designed paramagnetic enzymatic substrate to map alkaline phosphatase activity with a high selectivity, thereby demonstrating the potential of EPRI to map enzyme activity. |
| Keywords: | enzymes EPR spectroscopy imaging nitroxide phosphatase |