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Single‐Nanoparticle Cell Barcoding by Tunable FRET from Lanthanides to Quantum Dots |
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| Authors: | Chi Chen Dr. Lijiao Ao Yu‐Tang Wu Vjona Cifliku Dr. Marcelina Cardoso Dos Santos Emmanuel Bourrier Dr. Martina Delbianco Prof. David Parker Dr. Jurriaan M. Zwier Dr. Liang Huang Prof. Niko Hildebrandt |
| Affiliation: | 1. NanoBioPhotonics, Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, Université Paris-Sud, CNRS, CEA, Orsay, France;2. Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, P. R. China;3. Cisbio Bioassays, Codolet, France;4. Department of Chemistry, Durham University, Durham, UK;5. Current affiliation: Max Planck Institute of Colloids and Interfaces, Potsdam, Germany;6. College of Chemical Engineering, Zhejiang University of Technology, Hangzhou, P. R. China;7. nanofret.com |
| Abstract: | Fluorescence barcoding based on nanoparticles provides many advantages for multiparameter imaging. However, creating different concentration‐independent codes without mixing various nanoparticles and by using single‐wavelength excitation and emission for multiplexed cellular imaging is extremely challenging. Herein, we report the development of quantum dots (QDs) with two different SiO2 shell thicknesses (6 and 12 nm) that are coated with two different lanthanide complexes (Tb and Eu). FRET from the Tb or Eu donors to the QD acceptors resulted in four distinct photoluminescence (PL) decays, which were encoded by simple time‐gated (TG) PL intensity detection in three individual temporal detection windows. The well‐defined single‐nanoparticle codes were used for live cell imaging and a one‐measurement distinction of four different cells in a single field of view. This single‐color barcoding strategy opens new opportunities for multiplexed labeling and tracking of cells. |
| Keywords: | FRET imaging lanthanides photoluminescence lifetimes quantum dots |