Proximity Ligation‐Based Fluorogenic Imaging Agents for Neuraminidases |
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| Authors: | Dr Zhizeng Gao Dr Andrew J Thompson Prof James C Paulson Prof Stephen G Withers |
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| Institution: | 1. Department of Chemistry, University of British Columbia, Vancouver, British Columbia, Canada;2. Current Address: Marine Science Department, Sun Yat-san University, Zhuhai, Guangdong, China;3. Departments of Molecular Medicine and Microbiology and Immunology, The Scripps Research Institute, La Jolla, CA, USA |
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| Abstract: | Reagents to visualize and localize neuraminidase activity would be valuable probes to study the role of neuraminidases in normal cellular processes as well as during viral infections or cancer development. Herein, a new class of neuraminidase‐imaging probes that function as proximity ligation reagents by releasing a highly reactive fluorophore that tags nearby cellular material is described. It is further demonstrated that it is possible to create an influenza virus‐specific reagent, which can specifically detect influenza virus infections in mammalian cells. These reagents have potential use as specific histological probes independent of viral antigenicity and, therefore, offer some advantages over commonly used anti‐neuraminidase antibodies. |
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| Keywords: | fluorogenic probes histological imaging influenza virus neuraminidase |
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