A Photoactivatable BODIPY Probe for Localization‐Based Super‐Resolution Cellular Imaging |
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| Authors: | Chamari S Wijesooriya Julie A Peterson Pradeep Shrestha Elizabeth J Gehrmann Prof?Dr Arthur H Winter Prof?Dr Emily A Smith |
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| Institution: | 1. Department of Chemistry, Iowa State University, Ames, IA, USA;2. The Ames Laboratory, US Department of Energy, Ames, IA, USA |
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| Abstract: | The synthesis and application of a photoactivatable boron‐alkylated BODIPY probe for localization‐based super‐resolution microscopy is reported. Photoactivation and excitation of the probe is achieved by a previously unknown boron‐photodealkylation reaction with a single low‐power visible laser and without requiring the addition of reducing agents or oxygen scavengers in the imaging buffer. These features lead to a versatile probe for localization‐based microscopy of biological systems. The probe can be easily linked to nucleophile‐containing molecules to target specific cellular organelles. By attaching paclitaxel to the photoactivatable BODIPY, in vitro and in vivo super‐resolution imaging of microtubules is demonstrated. This is the first example of single‐molecule localization‐based super‐resolution microscopy using a visible‐light‐activated BODIPY compound as a fluorescent probe. |
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| Keywords: | BODIPY fluorescence imaging agents photoactivation super-resolution microscopy |
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