吸入白细胞介素-5对支气管哮喘患者外周血嗜酸性粒细胞数及其活化状态的影响

随机选择８例哮喘患者雾化吸入人重组ＩＬ－５，分别吸入前，吸入后２ｈ，２４ｈ和４８ｈ进行外周血有核细胞计烽和分类，以放射免疫法测定血清中嗜酸性阳离子蛋白（ＥＣＰ）及总ＩｇＥ水平，结果，吸入ＩＬ－５后嗜酸性粒细胞（ＥＯＳ）占细胞总数的百分比，ＥＯＳ绝对细胞数以及ＥＣＰ水平均随时间而明显升高，至２４ｈ达最高值，４８ｈ后仍维持在较高水平，ＩＬ－５在整个实验过程中对血清总ＩｇＥ水平无明显影响，表明，吸入ＩＬ

Effect of Inhaled Interleukin-5 on Activity and Number of Eosinophils in Circulation from Asthmatics

For examining the effects of recombinant human(rh) IL-5 inhalation on changes of activity and number of circulating eosinophils in allergic asthmatics, as well as concentrations of serum IgE,a randomized double-blind, placebo-controlled study design was employed, in which each subject acted as his or her own control. Eight nonsmoking patients with allergic asthma were administered rhIL-5 by nebulization, total blood nuclear cell counts and differentials, as well as concentrations of ECP and IgE in serum were determined before, and at 2 h,24 h,48 h after inhalation, respectively.Eosinophil numbers and ECP levels within the control group did not appear to change from baseline at any time throughout the study, eosinophil numbers from baseline(3.6±1.1×10⁵/mL) to 6.3±1.2×10⁵/mL (P<0.01) at 24 h,and to 5.7±0.9×10⁵/mL(P<0.01) at 48h after IL-5 inhalation. Accompanying this significant blood eosinophilia was a significant elevation of serum ECP levels. Compared with baseline value(6.3±1.1 pg/mL), inhalation of IL-5 lead to ECP levels increase with time, reaching 17.6±2.8 pg/mL at 24 h (P<0.01); and this elevated ECP levels lasted at least 48 h(18.1±2.9 pg/mL, P<0.01).IL-5 inhalation had no significant effect of levels of serum total IgE.our findings provided direct evidence that IL-5 not only induced a significant blood eosinophilia, but also resulted in the activation of circulating eosinophils.