One-step preparation of chitosan-coated cationic liposomes by an improved supercritical reverse-phase evaporation method |
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Authors: | Otake Katsuto Shimomura Takeshi Goto Toshihiro Imura Tomohiro Furuya Takeshi Yoda Satoshi Takebayashi Yoshihiro Sakai Hideki Abe Masahiko |
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Affiliation: | Nanotechnology Research Institute and Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8565, Japan. katsuto-otake@aist.go.jp |
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Abstract: | High-pressure carbon dioxide in contact with water dissolves to form carbonic acid, causing a decrease in pH. By use of these characteristics of a CO2/H2O biphasic system, chitosan-coated cationic liposomes of l-alpha-dipalmitoylphosphatidylcholine were successfully prepared by an improved supercritical reverse-phase evaporation (ISCRPE) method. Liposome-chitosan complexes carrying a positive charge were prepared in a single-step procedure without the use of acid or organic solvent, including ethanol. The maximum trapping efficiency of liposomes prepared by the ISCRPE method was 17%, with or without the addition of chitosan, compared to only 2% for liposomes prepared by the Bangham method. Furthermore, the liposomal dispersion was stable at room temperature in a sealed tube for over 30 days. |
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