高效液相色谱光度法测定痕量铜、锌、铁、锰的磺化四苯基卟啉配合物

本文用磺化四苯基卟啉作柱前衍生试剂,研究了试剂与铜(Ⅱ)、锌(Ⅱ)、铁(Ⅲ)及锰(Ⅱ)的络合反应。在ODS柱上,用乙腈-水(40:60,V/V)作流动相,溴化四丁基铵作离子对试剂,在420nm波长处进行光度检测。提出了离子对反相高效液相色谱快速分离及测定痕量铜、锌、铁及锰的新方法。检测下限为(×10^-3ppm):Mn 3.6、Fe 1.8 、Zn 0.93、Cu 0.70。应用于植物样品中痕量铜、锌、铁及锰的测定,分析结果与标准参考值相符。

Separation and Determination of Trace Amounts of Copper,Zinc,Iron andManganese with meso-Tetra-(4-Sulfophenyl)porphyrin Complexesby High Performance Liquid Chromatography

In this paper, chelating reactions of meso-tetra-(4-sulfophenyl)porphyrin(TPPS₄) used as the derivation agent in front of the column with Cu(Ⅱ),Zn(Ⅱ),Fe(Ⅲ) and Mn(Ⅱ ) have been studied. The chelates were separated on a ODScolumn(5 μm,200℃ 4. 6 mm i. d.) and eluted with acetonitrile-water(40 : 60,V/V,mobile phase) containing 10 mmol/Ltetrabutylammonium bromide (ion pair reagent) and 10 mmol/Lacetic acid-sodium acetate buffer(pH 4.1), and detected at 420 nm. A new method for rapid separation and determination of trace amounts of Cu,Zn,Fe and Mn by reversed-phase ion-pair HPLCis proposed. The detection limits (×10^-3 ppm) are 3. 6, 0. 93, 0. 70 and 1, 8 for Mn, Zn, Cu and Fe respectively.This method has been successfully applied to the determination of Cu, Zn, Fe and Mn in the standard sample of peach leaves. The analytical results are in agreement with standard reference values.