Rapid and quantitative determination of 10 major active components in Lonicera japonica Thunb. by ultrahigh pressure extraction-HPLC/DAD |
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| Authors: | Li Fan Changhu Lin Wenjuan Duan Xiao Wang Jianhua Liu |
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| Institution: | 1. Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, People's Republic of China;2. College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, People's Republic of China;3. Guizhou Research Center of Physical Test and Chemical Analysis, Guizhou Academy of Sciences, Guiyang 550002, People's Republic of China |
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| Abstract: | An ultrahigh pressure extraction (UPE)-high performance liquid chromatography (HPLC)/diode array detector (DAD) method was established to evaluate the quality of Lonicera japonica Thunb. Ten active components, including neochlorogenic acid, chlorogenic acid, 4-dicaffeoylquinic acid, caffeic acid, rutin, luteoloside, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, and quercetin, were qualitatively evaluated and quantitatively determined. Scanning electron microscope images elucidated the bud surface microstructure and extraction mechanism. The optimal extraction conditions of the UPE were 60% methanol solution, 400?MPa of extraction pressure, 3?min of extraction time, and 1:30 (g/mL) solid:liquid ratio. Under the optimized conditions, the total extraction yield of 10 active components was 57.62?mg/g. All the components showed good linearity (r2 ≥ 0.9994) and recoveries. This method was successfully applied to quantify 10 components in 22 batches of L. japonica samples from different areas. Compared with heat reflux extraction and ultrasonic-assisted extraction, UPE can be considered as an alternative extraction technique for fast extraction of active ingredient from L. japonica. |
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| Keywords: | Lonicera japonica Thunb ultrahigh pressure extraction ten active components HPLC/DAD SEM |
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