A novel silica-based column packing for reversed-phase HPLC of basic compounds |
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Authors: | Xiaojia Huang Junde Wang Xueliang Liu Zhenhua Shang |
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Affiliation: | (1) Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 161 Zhongshan Road, 116012 Dalian, People's Republic of China |
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Abstract: | Summary A novel bonded phase for reversed-phase HPLC was synthesized in two steps. Octylamine was first reacted with β-(3,4-epoxycyclohexyl)ethyltrimethoxysilane (β-ECTS) and then the intermediate product was coupled onto porous silica. The prepared packing was characterized by elemental analysis, solid-state13C NMR and Fourier transform infrared (FT-IR). Chromatographic evaluations were carried out by using a mixture of organic compounds including acidic, basic and neutral analytes and methanol-water as binary mobile phase. The results showed that the stationary phase has excellent chromatographic properties and is resistant to hydrolysis between pH=2≈8. It can be used efficiently for the separation of basic compounds. |
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Keywords: | Column liquid chromatography Reversed phase packing Basic compounds separation |
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