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Isolation and purification of endo-polygalacturonase by affinity chromatography in a fluidized bed reactor
Affiliation:1. Environmental and Chemical Technology Group, Department of Chemistry, Federal University of Ouro Preto, Campus Universitário Morro do Cruzeiro, Bauxita s/n, Ouro Preto 35400-000, Brazil;2. Universidad Antonio Nariño, Environmental and Civil Engineering Faculty, Bogotá, Colombia;1. Institute of Advanced Synthesis, School of Chemistry and Molecular Engineering, Jiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing Tech University, Nanjing 211816, China;2. Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Nanjing Tech University (Nanjing Tech), 30 South Puzhu Road, Nanjing 211800, China;3. The First Affiliated Hospital of Shenzhen University, Health Science Center, China;1. The University of Calabria – Department of Environment and Chemical Engineering, Via Pietro BUCCI, Cubo 44A, 87036 Rende, CS, Italy;2. National Research Council – Institute on Membrane Technology (ITM-CNR), Via Pietro BUCCI, Cubo 17C, 87036 Rende, CS, Italy
Abstract:Endo-polygalacturonase can be purified and isolated from an industrial pectolytic enzyme preparation using alginate as an affinity adsorbent in a fluidThe adsorption and desorption kinetics of the system have been studied. First the adsorption and desorption conditions were optimized with respect to pThe stability of the beads was tested in repeated adsorption/desorption experiments in a batch fluidized bed reactor isolating the enzyme from an indus
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