Electromicrobial transformations using the pyruvate synthase system of Clostridium sporogenes |
| |
| Institution: | 1. ProdAl Scarl, Competence Center on Agro-Food Productions, Via Ponte don Melillo, 84084, Fisciano, SA, Italy;2. Research Laboratories in Sciences Applied to Food, Canadian Irradiation Center, INRS-Institut Armand-Frappier, Institute of Nutraceutical and Functional Foods, 531, Boulevard des Prairies, Laval, Québec, Canada, H7V 1B7;3. Department of Industrial Engineering, University of Salerno, Via Giovanni Paolo II 132, 84084, Fisciano, SA, Italy;1. Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, 3010, Australia;2. Department of Food Science and Technology, Oregon State University, Corvallis, OR, 97331, USA;3. School of Public Health, Curtin Health Innovation Research Institute, Curtin University, Perth, WA, 6845, Australia |
| |
| Abstract: | A bioelectrochemical method by which the enzymology of reductive carboxylations (RCOOH + CO3 + 6 H] → RCH2COOH + 2 H2O) could be investigated is described. This method was used for a detailed study of the enyzmology of the overall reaction (viz. acetyl phosphate to pyruvate) catalysed by pyruvate synthase in Clostridium sporogenes. The same method could be utilised to harness such reductive carboxylations for commercial biotransformations of xenobiotics. By adjusting the reaction conditions it was possible to alter the proportions of the products synthesised, and to synthesise compounds more reduced and/or with a greater number of carbon atoms than pyruvate. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
