PEROXIDASE INDUCED CHEMILUMINESCENCE IN THE OXIDATION OF PYROGALLOL AND PURPUROGALLIN. INFLUENCE OF AZIDE |
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Authors: | Mirjam Halmann Gavriella Gabor Baruch Velan |
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Institution: | Israel Institute for Biological Research, P.O. Box 19, Ness-Ziona, Israel |
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Abstract: | Abstract— The chemiluminescence (CL) emitted during the peroxidase catalyzed oxidation of purpurogallin and pyrogallol depends on one and two molecules of enzyme, respectively. Using microperoxidase, CL with purpurogallin also depends on two molecules of enzyme (above 0.3 μ M enzyme). Sodium azide inhibits the CL emitted by all reactions depending on two molecules of enzyme but enhances the CL of reactions depending on one molecule of peroxidase (purpurogallin + 0.75 n M horseradish peroxidase). The formation of functional enzyme dimols during the oxidative process is discussed. |
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