Intrinsic Turn-On Response of Thioflavin T in Complexes |
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| Authors: | Dr Jocky C K Kung Dr Nesrin Vurgun JoAnn C Chen Prof Dr Mark Nitz Prof Dr Rebecca A Jockusch |
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| Institution: | 1. Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, M5S 3H6 Canada
These authors contributed equally to this work.;2. Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, M5S 3H6 Canada |
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| Abstract: | The fluorescence enhancement (“turn-on”) response of the amyloid-sensing dye thioflavin T (ThT) is examined in vacuo, where solvent interactions are absent. Upon the complexation of ThT with a derivatized β-cyclodextrin, heptakis-6-deoxy-6-(3-sulfanylpropanoic acid)]-β-cyclodextrin, turn-on responses in both the gas phase and solution phase were observed. In contrast, turn-on response was not detected when ThT was bound to gaseous cucurbit7]uril or human telomeric DNA 22AG, whereas clear turn-on response occurs in solution. The observed difference in turn-on response in the gas phase emphasizes the key interplay between chromophore, host and solvent and demonstrates the utility of gas-phase spectroscopy to tease out the balance among intermolecular forces driving the behavior of important chromophores in solution. |
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| Keywords: | cyclodextrins fluorescent probes mass spectrometry photophysics thioflavin T |
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