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Electro-kinetically driven route for highly sensitive blood pathology on a paper-based device |
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| Authors: | Naresh Kumar Mani Sankha Shuvra Das Sayantan Dawn Suman Chakraborty |
| Institution: | 1. Department of Biotechnology, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal, Karnataka, India
Department of Mechanical Engineering, Indian Institute of Technology Kharagpur, Kharagpur, India These authors contributed equally to this work.;2. Department of Mechanical Engineering, Indian Institute of Technology Kharagpur, Kharagpur, India These authors contributed equally to this work.;3. Department of Mechanical Engineering, Jadavpur University, Kolkata, India;4. Department of Mechanical Engineering, Indian Institute of Technology Kharagpur, Kharagpur, India |
| Abstract: | Enhancing the sensitivity of colorimetric detection in paper-devices is a quintessential step in achieving frugal diagnosis. Here, we demonstrate an effective way of improving the detection sensitivity of paper-based devices, as mediated by electro-kinetic mechanisms. By directly employing blood plasma, we investigate the electro-kinetic clustering of glucose, a neutral molecule in paper devices. Under the influence of uniform electric field, dispersed glucose gets accumulated in the paper strips. Due to the combination of EOF and electrophoretic migration, we achieve twofold increase in the colour intensity for both normal and diabetic samples. This approach is robust and possesses better sensitivity than conventional colorimetric assays and can be easily extended to other body fluid based diagnosis. These results may turn out to be of profound importance in improving the quality of pathological diagnosis in low-cost paper-based point-of-care devices deployed in resource-limited settings. |
| Keywords: | Blood Clustering Electro-kinetics Pathology Sensitivity |