Aqueous Synthesis of Triphenylphosphine-Modified Gold Nanoparticles for Synergistic In Vitro and In Vivo Photothermal Chemotherapy |
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| Authors: | Dr Farah Benyettou Dr Anjana Ramdas Nair Yaereen Dho Dr Thirumurugan Prakasam Dr Renu Pasricha Jamie Whelan Prof Hassan Traboulsi Javed Mazher Prof Kirsten C Sadler Prof Ali Trabolsi |
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| Institution: | 1. New York University Abu Dhabi, PO Box 129188, Saadiyat Island, Abu Dhabi, United Arab Emirates;2. Department of Chemistry, King Faisal University, Al-Ahsa, 31982 Kingdom of Saudi Arabia;3. Department of Physics, King Faisal University, Al-Ahsa, 31982 Kingdom of Saudi Arabia |
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| Abstract: | Triphenylphosphine (TPP) surface-functionalized and F-108 Pluronic-stabilized gold nanoparticles (F-108@TPP-AuNPs) have been synthesized through a one-step approach, leading to well-defined (9.6±1.6 nm) and water-soluble nanoparticles by microwave heating an aqueous solution of TPP-AuICl in the presence of a Pluronic polymer under basic conditions. TPP release was negligible under physiological conditions, but enhanced significantly at an acidic pH (5.4) mimicking that of a cancer cell. Laser irradiation (532 nm) raised the temperature of an aqueous solution of F-108@TPP-AuNPs to 51.7 °C within 5 min, confirming efficient light-to-heat conversion capabilities without significant photodegradation. TEM confirmed intracellular localization of F-108@TPP-AuNPs in the cytosol, endosomes and lysosomes of HeLa cells. F-108@TPP-AuNPs were well tolerated by HeLa cells and zebrafish embryos at ambient temperatures and became toxic upon heat activation, suggesting synergistic interactions between heat and cytotoxic action by TPP. |
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| Keywords: | apoptosis chemotherapy drug delivery system hyperthermia zebrafish |
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