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Evaluation of polymorphisms in repair and detoxification genes in alcohol drinkers and non-drinkers using capillary electrophoresis |
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| Authors: | Caroline Oliveira de Araújo Melo Thaís Cidália Vieira Marc Alexandre Duarte Gigonzac Jakeline Soares Fortes Sabrina Sara Moreira Duarte Aparecido Divino da Cruz Daniela de Melo e Silva |
| Institution: | 1. Federal University of Goiás, Goiânia, GO, Brazil
Postgraduate Program in Genetics/Replicon Research Center, Pontifical Catholic University of Goiás, Goiânia, GO, Brazil;2. Postgraduate Program in Genetics/Replicon Research Center, Pontifical Catholic University of Goiás, Goiânia, GO, Brazil;3. Federal University of Goiás, Goiânia, GO, Brazil Postgraduate Program in Genetics/Replicon Research Center, Pontifical Catholic University of Goiás, Goiânia, GO, Brazil Laboratory of Human Cytogenetics and Molecular Genetics, Goiás State Health Secretary, Goiânia, GO, Brazil;4. Federal University of Goiás, Goiânia, GO, Brazil |
| Abstract: | Alcohol use disorder (AUD) causes about 3.3 million deaths around the world each year. It is the primary risk factor for the global burden of diseases in American countries. Long-term abuse of alcohol induces numerous molecular and biochemical changes in tissues exposed to alcohol. The toxic effects of alcohol are mediated by DNA damage through various mechanisms, such as induction of oxidative damage, DNA adducts, crosslinks, and DNA strand breaks. The main aim of the current study was to compare the frequency of SNP polymorphisms in XRCC1 (rs7997782) and GSTP1 (rs1695) genes involved in DNA repair of single strand breaks (SSB) and xenobiotic detoxification between alcohol addicts and a control group comprised of non-drinkers. Genetic polymorphisms were identified following allelic specific PCR designed to generate the amplicons containing the variants. Then amplicons were sequenced, and sequences were aligned against the human genome reference deposited in GenBank using the CLC Sequence Viewer software (version 7.6.1). The GG homozygotes in rs1695 (GSTP1) were significantly (p = 0.023) 3.8x more frequent among those with AUD when compared to the control group. No SNP variation was observed in rs7997782 (XRCC1). rs1695 variant has been associated with susceptibility to various diseases, including those related to alcohol consumption. |
| Keywords: | Alcohol DNA damage Polymorphisms Xenobiotics |