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Strategies for 1H-Detected Dynamic Nuclear Polarization Magic-Angle Spinning NMR Spectroscopy |
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| Authors: | Dr Maria Concistré Dr Subhradip Paul Marina Carravetta Ilya Kuprov Dr Philip T F Williamson |
| Affiliation: | 1. School of Chemistry, University of Southampton, Southampton, SO171BJ UK;2. Sir Peter Mansfield Imaging Centre, University of Nottingham, Nottingham, UK;3. Biological Sciences/Institute of Life Sciences, University of Southampton, Southampton, SO171BJ UK |
| Abstract: | Combining dynamic nuclear polarization with proton detection significantly enhances the sensitivity of magic-angle spinning NMR spectroscopy. Herein, the feasibility of proton-detected experiments with slow (10 kHz) magic angle spinning was demonstrated. The improvement in sensitivity permits the acquisition of indirectly detected 14N NMR spectra allowing biomolecular structures to be characterized without recourse to isotope labelling. This provides a new tool for the structural characterization of environmental and medical samples, in which isotope labelling is frequently intractable. |
| Keywords: | 14N NMR spectroscopy amyloids biomolecular NMR spectroscopy dynamic nuclear polarization solid-state NMR spectroscopy |