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Supramolecular Regulation of Polydopamine Formation by Amyloid Fibers |
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| Authors: | J H Shin Nghia T K Le Prof?Dr Hongje Jang Dr Taehoon Lee Prof?Dr Kyungtae Kang |
| Institution: | 1. Department of Applied Chemistry, Kyung Hee University, 1732 Deogyoung-daero, Yongin, Gyeonggi, 17104 Republic of Korea
These authors contributed equally to this work.;2. Department of Chemistry, Kwangwoon University, 20 Gwangwoon-ro, Nowon-gu, Seoul, 01897 Republic of Korea;3. Department of Applied Chemistry, Kyung Hee University, 1732 Deogyoung-daero, Yongin, Gyeonggi, 17104 Republic of Korea |
| Abstract: | Polydopamine (PD) and melanin species are chemically complex systems, the formation and properties of which are incompletely understood. Inspired by the role of functional amyloids in melanin biosynthesis, this paper examines the influences of the supramolecular structure of amyloids on oxidative polymerization of dopamine. Kinetic analyses on the formation of PD species in the presence of hen egg white lysozyme (HEWL) fibers or soluble HEWL revealed that both forms gave rise to the total quantity of PD species, but the rate of their formation could be accelerated only by the amyloid form. PD species formed with HEWL fibers showed a morphology of bundled fibers, whereas those with soluble HEWL had a mesh-like structure. Amyloid fibers of recombinant Pmel17 had properties similar to those of HEWL fibers in modulating PD formation. The results presented here suggest how nature designs functionality with an amyloid structure and can help understand and engineer chemistries of other functional amyloids. |
| Keywords: | amyloid beta-peptides bioorganic chemistry melanin biosynthesis peptides polydopamine |