Role of anti-inflammatory interventions in high-fat-diet-induced obesity |
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Authors: | Rasha Abdelmawla Ghazala Azza El Medney Anisa Meleis Passant Mohie El dien Hend Samir |
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Affiliation: | 1. Medical Biochemistry Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt;2. Clinical Pharmacology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt;3. Histology and Cell Biology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt |
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Abstract: | Lipotoxicity is defined as deposition of excess fat associated with an inflammatory response. Metabolomic analysis of fatty acids (FAs) can be a marker of silent inflammation. ω3-Enriched diet, celecoxib, and safranal may have a protective anti-inflammatory role. In this work, total FAs extracted from red blood cells and arachidonic acid-to-eicosapentaenoic acid (AA-to-EPA) ratios were assessed using GC–MS assay in single-ion monitoring mode. The study was conducted on 64 male rats divided into eight groups: I, controls; II, rats received high-fat diet (HFD), III, rats received ω-6-enriched HFD; IV, rats received ω-3-enriched HFD; V, rats received celecoxib with HFD; VI, rats received safranal with HFD; VII and VIII, rats received celecoxib and safranal with ω-3 HFD, respectively. GC–MS Gas chromatography Mass spectrometry was performed for analysis of fatty acid methyl ester. Enzyme-linked immunosorbent assay was used to analyze serum interleukin-6 (IL-6) and transforming growth factor-beta 1 (TGF-β1) concentrations. A statistically significant decrease of AA-to-EPA ratio was observed in group VII when compared with the groups receiving HFDs. This group also showed the lowest serum IL-6 level and highest TGF-β1 level. In conclusion, ω3-enriched diet along with drugs (e.g. celecoxib) and herbal medications (e.g. safranal) may have an anti-inflammatory effect in lipotoxicity. GC–MS with single-ion monitoring is valid for the analysis of FAs. |
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Keywords: | arachidonic acid/eicosapentaenoic acid gas chromatographic-mass spectral IL-6 lipotoxicity TGF-β1 |
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