A volatolomic approach using cerumen as biofluid to diagnose bovine intoxication by Stryphnodendron rotundifolium |
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Authors: | João Marcos G Barbosa Milenna K Fernandes Rodrigues Lurian C David Taynara C e Silva Danielly A Fortuna Lima Naiara Z Pereira Emmanuel B D'Alessandro Anselmo E de Oliveira Paulo H Jorge da Cunha Maria Clorinda S Fioravanti Nelson R Antoniosi Filho |
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Affiliation: | 1. Laboratory of Extraction and Separation Methods (LAMES), Institute of Chemistry, Federal University of Goiás (UFG), Goiânia, GO, Brazil;2. Veterinary and Zootechnical School (EVZ), Federal University of Goiás (UFG), Goiânia, GO, Brazil;3. Laboratory of Theoretical and Computational Chemistry (LQTC), Institute of Chemistry, Federal University of Goiás (UFG), Goiânia, GO, Brazil |
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Abstract: | An innovative volatolomic approach employs the detection of biomarkers present in cerumen (earwax) to identify cattle intoxication by Stryphnodendron rotundifolium Mart., Fabaceae (popularly known as barbatimão). S. rotundifolium is a poisonous plant with the toxic compound undefined and widely distributed throughout the Brazilian territory. Cerumen samples from cattle of two local Brazilian breeds (‘Curraleiro Pé-Duro’ and ‘Pantaneiro’) were collected during an experimental intoxication protocol and analyzed using headspace (HS)/GC–MS followed by multivariate analysis (genetic algorithm for a partial least squares, cluster analysis, and classification and regression trees). A total of 106 volatile organic metabolites were identified in the cerumen samples of bovines. The intoxication by S. rotundifolium influenced the cerumen volatolomic profile of the bovines throughout the intoxication protocol. In this way, it was possible to detect biomarkers for cattle intoxication. Among the biomarkers, 2-octyldecanol and 9-tetradecen-1-ol were able to discriminate all samples between intoxicated and nonintoxicated bovines. The cattle intoxication diagnosis by S. rotundifolium was accomplished by applying the cerumen analysis using HS/GC–MS, in an easy, accurate, and noninvasive way. Thus, the proposed bioanalytical chromatography protocol is a useful tool in veterinary applications to determine this kind of intoxication. |
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Keywords: | biomarkers classification and regression trees cluster analysis genetic algorithm for a partial least squares HS/GC–MS volatile organic metabolites |
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