Accurate and simple determination of oxcarbazepine in human plasma and urine samples using switchable-hydrophilicity solvent in GC–MS |
| |
Authors: | Sezin Erarpat Süleyman Bodur Merve Fırat Ayyıldız Ömer Tahir Günkara Fatih Erulaş Dotse Selali Chormey Fatma Turak Türkan Börklü Budak Sezgin Bakırdere |
| |
Institution: | 1. Yıldız Technical University, Faculty of Art and Science, Chemistry Department, Istanbul, Turkey;2. Siirt University, Faculty of Education, Department of Science Education, Siirt, Turkey |
| |
Abstract: | This work presents a sensitive and rapid analytical method for the determination of oxcarbazepine in human plasma and urine samples. A vortex-assisted switchable hydrophilicity solvent-based liquid phase microextraction (VA–SHS–LPME) was used to preconcentrate oxcarbazepine from the samples before the determination by gas chromatography mass spectrometry. The switchable hydrophilicity solvent was synthesized by protonating N,N-dimethylbenzylamine with carbon dioxide to make it totally miscible with an equivalent volume of water. Parameters of the VA–SHS–LPME method including volume of switchable hydrophilicity solvent, concentration/volume of sodium hydroxide and vortex period were systematically optimized. Under the optimum conditions, good linearity ranging from 27.03 to 353.47 μg/kg was obtained for the analyte. Limit of detection and quantitation values were found to be 6.2 and 21 μg/kg (mass base), respectively. The relative standard deviation was calculated as 6.9% for six replicate measurements of the lowest concentration of the calibration plot. Satisfactory recovery results were calculated in the range of 97–100% for human plasma and urine samples spiked at five different concentrations. |
| |
Keywords: | gas chromatography–mass spectrometry human plasma oxcarbazepine switchable hydrophilicity solvent urine |
|
|