Development and validation of an LC–MS/MS method for the quantitative analysis of the adenosine A2a receptor antagonist NIR178 and its monohydroxy metabolite in human plasma: Application to clinical pharmacokinetics |
| |
Authors: | Olivier Heudi Nathalie Plaud Celine Aumonier Shari Wu Panos Hatsis Felipe K Hurtado Franck Picard Serge Winter Jimmy Flarakos |
| |
Institution: | 1. Novartis Pharma AG, PK Sciences/Bioanalytics, Basel, Switzerland;2. SGS, Saint-Benoît, France;3. Pharmacokinetic Sciences-Safety & ADME Bioanalysis, Novartis Institutes for BioMedical Research, One Health Plaza, East Hanover, NJ, USA;4. Oncology Clinical Pharmacology, Novartis Pharmaceuticals Corporation, East Hanover, New Jersey, USA |
| |
Abstract: | We report a selective LC–MS/MS method for the simultaneous quantitative determinations of the adenosine A2a receptor antagonist NIR178 (NIR178) and its major metabolite NJI765 in human plasma. Sample preparation steps involved protein precipitation, sample evaporation and reconstitution using a plasma sample volume of 0.1 ml plasma. Separation was achieved in 10 min on an Acquity UPLC BEH C18 1.7 μm, 2.1 × 50 mm column heated at 60°C with a gradient elution at 0.6 ml/min mobile phase made of water and acetonitrile both acidified with 0.1% formic acid. The detection was performed in positive ion mode and quantification based on multiple reaction monitoring. The linear response range was 1.00–1,000 ng/ml using a 1/x2 weighting factor. The intra- and inter-day accuracies (bias %) and intra- and inter-day precisions (CV, %) obtained for NIR178 and NJI765 were within the acceptance criteria. The normalized NIR178 and NJI765 matrix factor calculated from six lots of normal, lipemic and hemolyzed plasmas ranged from 0.97 to 1.05. The normalized recoveries of both NIR178 and NJI765 compared with their internal standards were consistent and reproducible with a CV ≤8.0. This method was successfully applied to support pharmacokinetic studies in adult patients with cancer. |
| |
Keywords: | adenosine A2a receptor antagonists clinical pharmacokinetics immuno-oncology LC–MS/MS NIR178 validation |
|
|