| Synthesis, DNA-binding and photocleavage studies of ruthenium(Ⅱ) complexes [Ru(btz)_3]~(2+) and [Ru(btz)(dppz)_2]~(2+) |
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| 基金项目： | Supported by the 973 Program of China (Grant No 2007CB815306);;the National Natural Science Foundation of China (Grant Nos 20771105 & 20871122);;the Pro-gram for New Century Excellent Talents in University (Grant No NCET-06-0718);;the Key Project (Grant No 108103) of Ministry of Education;;the Natural Sci-ence Foundation of Guangclong Province (Grant No 9351027501000003) |
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| 摘 要： | Two new ruthenium(Ⅱ) complexes, Ru(btz)3](ClO4)2 (1) and Ru(btz)(dppz)2](ClO4)2 (2) (btz = 4,4′-bithi-azole, dppz = dipyrido3,2-a:2′,3′-c]phenazine), have been synthesized and characterized by elemental analysis, 1H NMR, ES-MS and X-ray crystallography. The DNA binding behaviors of two complexes have been studied by spectroscopic and viscosity measurements. The results suggest that complex 1 binds to CT-DNA via an electrostatic mode, while complex 2 via an intercalative mode. Under irradiation at 365 nm,...
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| 关 键 词： | ruthenium（Ⅱ） complex DNA binding DNA photocleavage PDT |
| 收稿时间： | 22 February 2010 |
Synthesis, DNA-binding and photocleavage studies of ruthenium(II) complexes [Ru(btz)3]2+ and [Ru(btz)(dppz)2]2+ |
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| Authors: | HuiJuan Yu ShuMei Huang JunFeng Kou LvYing Li HaiNa Jia Hui Chao and LiangNian Ji |
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| Institution: | YU HuiJuan,HUANG ShuMei,KOU JunFeng,LI LvYing,JIA HaiNa,CHAO Hui & JI LiangNian MOE Laboratory of Bioinorganic , Synthetic Chemistry,School of Chemistry , Chemical Engineering,Sun Yat-Sen University,Guangzhou 510275,China |
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| Abstract: | Two new ruthenium(II) complexes, Ru(btz)3](ClO4)2 (1) and Ru(btz)(dppz)2](ClO4)2 (2) (btz = 4,4′-bithiazole, dppz = dipyrido3,2-a:2′,3′-c]phenazine), have been synthesized and characterized by elemental
analysis, 1H NMR, ES-MS and X-ray crystallography. The DNA binding behaviors of two complexes have been studied by spectroscopic and
viscosity measurements. The results suggest that complex 1 binds to CT-DNA via an electrostatic mode, while complex 2 via
an intercalative mode. Under irradiation at 365 nm, both complexes were found to promote the cleavage of plasmid pBR 322 DNA
from supercoiled form I to nicked form II. The mechanism studies reveal that singlet oxygen 1O2 and hydroxyl radical (OH.) play a significant role in the photocleavage process. |
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