Screening of antinociceptive components in Corydalis yanhusuo W.T. Wang by comprehensive two-dimensional liquid chromatography/tandem mass spectrometry |
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Authors: | Chen Wang Shuowen Wang Guorong Fan Hanfa Zou |
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Institution: | (1) Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai, 200433, China;(2) Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian, 116023, China;(3) Shanghai Key Laboratory for Pharmaceutical Metabolite Research, 325 Guohe Road, Shanghai, 200433, China; |
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Abstract: | Formalin-induced pain models were used in rats to evaluate the antinociceptive effect of the total alkaloids of Corydalis yanhusuo (TAC). The results indicated that formalin-evoked spontaneous nociceptive responses (licking behavior) could be inhibited
significantly by giving (intragingival) TAC at a single dose of 150 mg/kg. Subsequently, an online comprehensive two-dimensional
biochromatography method with a silica-bonded human serum albumin (HSA) column in the first dimension and a monolithic ODS
column in the second was developed. The absorbed bioactive components were screened by comparing and contrasting the components
detected in the plasma and striatum with those in TAC. More than 100 compounds were separated and detected in the TAC, among
which 13 compounds were identified. About 40 compounds (seven compounds identified) were absorbed into the plasma with appropriate
concentrations and about 20 compounds (four compounds identified) passed through the blood-brain barrier into the striatum.
Of interest, four compounds (protopine, glaucine, tetrahydropalmatine, and corydaline) which were reported to possess profound
antinociceptive effects exhibited high concentrations in the striatum. This may result from their synergistic effects in regulating
the formalin-induced nociception. The results indicated that the comprehensive two-dimensional biochromatography method developed
is capable of screening the bioactive components in Corydalis yanhusuo and providing valuable information for understanding the mechanisms by which Corydalis yanhusuo alleviates nociception. |
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