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Microscopic visualization of alamethicin incorporation into model membrane monolayers
Authors:Volinsky Roman  Kolusheva Sofiya  Berman Amir  Jelinek Raz
Institution:Ilse Katz Center for Nano- and Mesoscience and Technology, Department of Chemistry, and Department of Biotechnology Engineering, Ben Gurion University of the Negev, Beer-Sheva 84105, Israel.
Abstract:Lipid interactions and cooperative assembly properties are fundamental determinants for the action of antimicrobial membrane-active peptides. Here we analyze the interactions and aggregation properties of alamethicin, an antimicrobial pore-forming peptide, with films formed at the air/water interface. Surface-area/pressure isotherms, Brewster angle microscopy, and fluorescence-confocal microscopy provided detailed information on the morphologies and structural properties of the peptide and its effect on the film components. The pressure-area analysis and microscopy experiments facilitated unprecedented visualization of the structural consequences of alamethicin association at the air/water interface, with pure phospholipid films, and within mixed phospholipid/polydiacetylene (PDA) films. The analysis exposed the kinetic features and the interplay between the peptide aggregates and film constituents. In particular, the results demonstrate the use of phospholipid/PDA film assemblies for studying membrane-peptide association and interactions within two-dimensional films.
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