| 尖吻蝮蛇毒抗凝血因子(ACF)含量和钙离子含量的定量测定及钙离子结合位点的初步研究 |
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| 引用本文： | 刘清亮,徐晓龙,余华明,王淳.尖吻蝮蛇毒抗凝血因子(ACF)含量和钙离子含量的定量测定及钙离子结合位点的初步研究[J].无机化学学报,1993,9(1):71-76. |
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| 作者姓名： | 刘清亮 徐晓龙 余华明 王淳 |
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| 作者单位： | 中国科技大学应用化学系, 合肥 230026,中国科技大学应用化学系, 合肥 230026,中国科技大学应用化学系, 合肥 230026,中国科技大学应用化学系, 合肥 230026;中国科技大学生物系 合肥 230026 |
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| 摘 要： | 由紫外光谱法测定蛋白质浓度,用ICP测定Ca含量,从而确定一个ACF分子中含一个钙离子.ACF中分子中除一个高亲和性钙结合位点外,至少还有一个低亲和性钙结合位点,只有当过量钙离子存在时,才可能在低亲和性钙结合位点上进一步结合钙离子.Tb3+具有比Ca2+离子更强的键合ACF能力,能定量结合在ACF中的两个位点上,且能全部取代ACF中Ca2+.文中提及的金属离子对ACF的抗凝血活性没有显著的影响.
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| 关 键 词： | 抗凝血因子 钙 铽 荧光 尖吻蝮蛇 |
| 收稿时间： | 1991/8/31 0:00:00 |
DETERMINATION OF THE CONTENTS OF THE Ca2+ ION IN ACF AND THE STUDY OF Ca2+-BINDING SITES |
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| Liu Qingliang,Xu Xiaolong,Yu Huaming and Wang Chun.DETERMINATION OF THE CONTENTS OF THE Ca2+ ION IN ACF AND THE STUDY OF Ca2+-BINDING SITES[J].Chinese Journal of Inorganic Chemistry,1993,9(1):71-76. |
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| Authors: | Liu Qingliang Xu Xiaolong Yu Huaming and Wang Chun |
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| Institution: | Department of Applied Chemistry, University of Science and Technology of China, Hefei 230026,Department of Applied Chemistry, University of Science and Technology of China, Hefei 230026,Department of Applied Chemistry, University of Science and Technology of China, Hefei 230026 and Department of Applied Chemistry, University of Science and Technology of China, Hefei 230026 |
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| Abstract: | The corrective coefficient of the equation on determination of the concentration of ACF by UV spectral (280nm-260nm) has been introduced. Our studies show that each ACF molecule contains one of Ca2+ ion, and has two Ca2+-binding sites at least (stronger one and weaker others). Ca2+ ion can bind to the weaker site in ACF only in the condition of existence of excessive Ca2+ ion. Tb3+ ion is coordinated with ACF more strongly than Ca2+ ion, while Tb3+ ion can bind to ACF at both two sites quantitatively and replace of Ca2+ ion in ACF completely. All the metal ions mentioned in this paper do not give intensive effect on the anticoagulation activity of ACF. |
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| Keywords: | anticoagulation factor calcium terbium fluorescence |
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