Optimization of the mobile phase composition in gradient elution reversed-phase HPLC by stochastic prediction |
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| Authors: | G D'Agostino M J O'Hare F Mitchell T Salomon F Vérillon |
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| Institution: | (1) Ecolab srl. Viale della Liberta 56, 90143 Palermo, Italy;(2) Institute for Cancer Reseach, Royal Cancer Hospital, SM2 SPX Sutton, Surrey, England;(3) Gilson Medical Electronics, 72 rue Gambetta, 95400 Villiers le Bel, France |
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| Abstract: | Summary Quantitative analysis of more than ten compounds in a sample generally requires complex mobile phases to optimize the separation
of the analytes by gradient elution reversed-phase HPLC. For this purpose, CHEOPS, a software package has been developed from
the fully stochastic Computer Chromatogram Simulation Method. Calculation principles and optimization criteria are described.
Experimental validation is presented with amino acids and steroids. |
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| Keywords: | RP-HPLC Mobile phase Optimization Gradient elution |
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